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dedicated don extraction buffer (R-Biopharm)

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R-Biopharm dedicated don extraction buffer

The general concept of <t>the</t> <t>ID-LFIA</t> DART (this work) and ESI methods. <t>DON</t> is extracted from grounded wheat, and the extract is diluted with the running buffer on a 1:5 ratio. If the screening result is positive, the same sample extract is diluted with the running buffer on a 1:1 to develop the ID-LFIA. The ID-LFIA can then be further processed in the lab by washing and dissociating the bound DON and conjugates from the mAb. The final step is the rapid direct analysis by the newly developed and validated semi-quantitative DART-QqQ-MS/MS and the previously developed ESI-QqQ-MS/MS or ESI-Q-Orbitrap-MS. In case further information is needed, e.g., absolute quantitation over a different range or multitoxin analysis, conventional time-consuming LC-MS/MS analysis may be considered a follow-up.

Dedicated Don Extraction Buffer, supplied by R-Biopharm, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dedicated+don+extraction+buffer/dedicated+don+extraction+buffer/pmc07962121-86-15-22
Average 90 stars, based on 1 article reviews

dedicated don extraction buffer - by Bioz Stars, 2026-09

90/100 stars

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1) Product Images from "From Smartphone Lateral Flow Immunoassay Screening to Direct MS Analysis: Development and Validation of a Semi-Quantitative Direct Analysis in Real-Time Mass Spectrometric (DART-MS) Approach to the Analysis of Deoxynivalenol"

Journal: Sensors (Basel, Switzerland)

doi: 10.3390/s21051861

The general concept of the ID-LFIA DART (this work) and ESI methods. DON is extracted from grounded wheat, and the extract is diluted with the running buffer on a 1:5 ratio. If the screening result is positive, the same sample extract is diluted with the running buffer on a 1:1 to develop the ID-LFIA. The ID-LFIA can then be further processed in the lab by washing and dissociating the bound DON and conjugates from the mAb. The final step is the rapid direct analysis by the newly developed and validated semi-quantitative DART-QqQ-MS/MS and the previously developed ESI-QqQ-MS/MS or ESI-Q-Orbitrap-MS. In case further information is needed, e.g., absolute quantitation over a different range or multitoxin analysis, conventional time-consuming LC-MS/MS analysis may be considered a follow-up.

Figure Legend Snippet: The general concept of the ID-LFIA DART (this work) and ESI methods. DON is extracted from grounded wheat, and the extract is diluted with the running buffer on a 1:5 ratio. If the screening result is positive, the same sample extract is diluted with the running buffer on a 1:1 to develop the ID-LFIA. The ID-LFIA can then be further processed in the lab by washing and dissociating the bound DON and conjugates from the mAb. The final step is the rapid direct analysis by the newly developed and validated semi-quantitative DART-QqQ-MS/MS and the previously developed ESI-QqQ-MS/MS or ESI-Q-Orbitrap-MS. In case further information is needed, e.g., absolute quantitation over a different range or multitoxin analysis, conventional time-consuming LC-MS/MS analysis may be considered a follow-up.

Techniques Used: Tandem Mass Spectroscopy, Quantitation Assay, Liquid Chromatography with Mass Spectroscopy

Comparison between full scan ( m / z 100–600) mass spectra of a MeOH/NH 3 2% v / v solution of DON containing 10% v / v LFIA assay running buffer in ( a ) negative and ( b ) positive ion mode in DART-Orbitrap-MS and ( c ) positive ion mode in ESI-Orbitrap-MS. Inserted are the regions of deprotonated and protonated ions of DON, and marked in red are the expected m / z regions for the ions at 5 ppm mass accuracy.

Figure Legend Snippet: Comparison between full scan ( m / z 100–600) mass spectra of a MeOH/NH 3 2% v / v solution of DON containing 10% v / v LFIA assay running buffer in ( a ) negative and ( b ) positive ion mode in DART-Orbitrap-MS and ( c ) positive ion mode in ESI-Orbitrap-MS. Inserted are the regions of deprotonated and protonated ions of DON, and marked in red are the expected m / z regions for the ions at 5 ppm mass accuracy.

Techniques Used:

DART helium gas temperature optimization in QqQ-MS/MS for DON (blue) and 3-AcDON (red) 60 ng/mL, in MeOH/NH 3 2% v / v (solid line) and MeOH/NH 3 2% v / v exposed to the ID-LFIA substrate (dotted line). Measurements were performed in duplicate by MRM monitoring of the ion transitions specified in . The standard deviation of the duplicate measurement is shown by the error bars. The results are presented as the relative (%) area intensity, i.e., the ratio of the absolute area intensity divided by the highest absolute area intensity observed for each substance in each solution.

Figure Legend Snippet: DART helium gas temperature optimization in QqQ-MS/MS for DON (blue) and 3-AcDON (red) 60 ng/mL, in MeOH/NH 3 2% v / v (solid line) and MeOH/NH 3 2% v / v exposed to the ID-LFIA substrate (dotted line). Measurements were performed in duplicate by MRM monitoring of the ion transitions specified in . The standard deviation of the duplicate measurement is shown by the error bars. The results are presented as the relative (%) area intensity, i.e., the ratio of the absolute area intensity divided by the highest absolute area intensity observed for each substance in each solution.

Techniques Used: Tandem Mass Spectroscopy, Standard Deviation

Results from ID-LFIA/DART-QqQ-MS/MS analysis and the respective LFIA smartphone screening of 21 validation blank and spiked wheat samples at three different target levels and performed on three different days.

Figure Legend Snippet: Results from ID-LFIA/DART-QqQ-MS/MS analysis and the respective LFIA smartphone screening of 21 validation blank and spiked wheat samples at three different target levels and performed on three different days.

Techniques Used:

( a ). Representative chronograms for DON-spiked wheat samples used to develop the ID-LFIA, retrieved in dissociation solution and analyzed by DART-QqQ-MS/MS, demonstrating the three distinct spiking levels in blue 0.5× TL, orange 1× TL, and green 1.5× TL. 13 C 15 DON is added as an internal standard before the MS/MS analysis. ( b ). Data analysis of 21 blank wheat samples, and spiked versions thereof in ID-LFIA/DART-QqQ-MS/MS, at three target level concentrations in blue 0.5× TL, orange 1× TL, and green 1.5× TL.

Figure Legend Snippet: ( a ). Representative chronograms for DON-spiked wheat samples used to develop the ID-LFIA, retrieved in dissociation solution and analyzed by DART-QqQ-MS/MS, demonstrating the three distinct spiking levels in blue 0.5× TL, orange 1× TL, and green 1.5× TL. 13 C 15 DON is added as an internal standard before the MS/MS analysis. ( b ). Data analysis of 21 blank wheat samples, and spiked versions thereof in ID-LFIA/DART-QqQ-MS/MS, at three target level concentrations in blue 0.5× TL, orange 1× TL, and green 1.5× TL.

Techniques Used: Tandem Mass Spectroscopy

( a ). Chronogram of AcDON-spiked wheat sample used to develop the ID-LFIA and retrieved in dissociation solution analyzed by DART-QqQ-MS/MS, demonstrating the three distinct TL in blue 0.5× TL, orange 1× TL, and green 1.5× TL. 13 C 15 DON is added as an internal standard before the MS analysis. ( b ). Chronogram of the incurred corn starch sample used to develop the ID-LFIA and retrieved in dissociation solution analyzed in A . (+)DART-QqQ-MS/MS and B . (−)ESI-QqQ-MS/MS in flow injection analysis mode. 13 C 15 DON is added as an internal standard prior to MS analysis.

Figure Legend Snippet: ( a ). Chronogram of AcDON-spiked wheat sample used to develop the ID-LFIA and retrieved in dissociation solution analyzed by DART-QqQ-MS/MS, demonstrating the three distinct TL in blue 0.5× TL, orange 1× TL, and green 1.5× TL. 13 C 15 DON is added as an internal standard before the MS analysis. ( b ). Chronogram of the incurred corn starch sample used to develop the ID-LFIA and retrieved in dissociation solution analyzed in A . (+)DART-QqQ-MS/MS and B . (−)ESI-QqQ-MS/MS in flow injection analysis mode. 13 C 15 DON is added as an internal standard prior to MS analysis.

Techniques Used: Tandem Mass Spectroscopy, Injection

Results from ID-LFIA/DART-QqQ-MS/MS analysis and the respective LFIA smartphone screening of AcDON-spiked wheat samples at three target levels and the analysis of an incurred corn starch sample.

Figure Legend Snippet: Results from ID-LFIA/DART-QqQ-MS/MS analysis and the respective LFIA smartphone screening of AcDON-spiked wheat samples at three target levels and the analysis of an incurred corn starch sample.

Techniques Used:

Extraction:

Article Title: From Smartphone Lateral Flow Immunoassay Screening to Direct MS Analysis: Development and Validation of a Semi-Quantitative Direct Analysis in Real-Time Mass Spectrometric (DART-MS) Approach to the Analysis of Deoxynivalenol
Article Snippet: .. For the screening assays, a smartphone-based LFIA kit, Rida Quick DON RQS Eco, including its dedicated DON extraction buffer, was obtained from r-Biopharm (Darmstadt, Germany). .. The MS/MS analysis was performed on a model Xevo TQ-XS Tandem Triple Quadrupole (QqQ) MS/MS system (Waters Corporation, Milford, MA, USA) equipped with a DART SVP ion source (IonSense, Saugus, MA, USA).

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Journal: Sensors (Basel, Switzerland)

doi: 10.3390/s21051861

Figure Lengend Snippet: The general concept of the ID-LFIA DART (this work) and ESI methods. DON is extracted from grounded wheat, and the extract is diluted with the running buffer on a 1:5 ratio. If the screening result is positive, the same sample extract is diluted with the running buffer on a 1:1 to develop the ID-LFIA. The ID-LFIA can then be further processed in the lab by washing and dissociating the bound DON and conjugates from the mAb. The final step is the rapid direct analysis by the newly developed and validated semi-quantitative DART-QqQ-MS/MS and the previously developed ESI-QqQ-MS/MS or ESI-Q-Orbitrap-MS. In case further information is needed, e.g., absolute quantitation over a different range or multitoxin analysis, conventional time-consuming LC-MS/MS analysis may be considered a follow-up.

Article Snippet: For the screening assays, a smartphone-based LFIA kit, Rida Quick DON RQS Eco, including its dedicated DON extraction buffer, was obtained from r-Biopharm (Darmstadt, Germany).

Techniques: Tandem Mass Spectroscopy, Quantitation Assay, Liquid Chromatography with Mass Spectroscopy

Journal: Sensors (Basel, Switzerland)

doi: 10.3390/s21051861

Figure Lengend Snippet: Comparison between full scan ( m / z 100–600) mass spectra of a MeOH/NH 3 2% v / v solution of DON containing 10% v / v LFIA assay running buffer in ( a ) negative and ( b ) positive ion mode in DART-Orbitrap-MS and ( c ) positive ion mode in ESI-Orbitrap-MS. Inserted are the regions of deprotonated and protonated ions of DON, and marked in red are the expected m / z regions for the ions at 5 ppm mass accuracy.

Article Snippet: For the screening assays, a smartphone-based LFIA kit, Rida Quick DON RQS Eco, including its dedicated DON extraction buffer, was obtained from r-Biopharm (Darmstadt, Germany).

Techniques:

Journal: Sensors (Basel, Switzerland)

doi: 10.3390/s21051861

Figure Lengend Snippet: DART helium gas temperature optimization in QqQ-MS/MS for DON (blue) and 3-AcDON (red) 60 ng/mL, in MeOH/NH 3 2% v / v (solid line) and MeOH/NH 3 2% v / v exposed to the ID-LFIA substrate (dotted line). Measurements were performed in duplicate by MRM monitoring of the ion transitions specified in . The standard deviation of the duplicate measurement is shown by the error bars. The results are presented as the relative (%) area intensity, i.e., the ratio of the absolute area intensity divided by the highest absolute area intensity observed for each substance in each solution.

Article Snippet: For the screening assays, a smartphone-based LFIA kit, Rida Quick DON RQS Eco, including its dedicated DON extraction buffer, was obtained from r-Biopharm (Darmstadt, Germany).

Techniques: Tandem Mass Spectroscopy, Standard Deviation

Journal: Sensors (Basel, Switzerland)

doi: 10.3390/s21051861

Figure Lengend Snippet: Results from ID-LFIA/DART-QqQ-MS/MS analysis and the respective LFIA smartphone screening of 21 validation blank and spiked wheat samples at three different target levels and performed on three different days.

Article Snippet: For the screening assays, a smartphone-based LFIA kit, Rida Quick DON RQS Eco, including its dedicated DON extraction buffer, was obtained from r-Biopharm (Darmstadt, Germany).

Techniques:

Journal: Sensors (Basel, Switzerland)

doi: 10.3390/s21051861

Figure Lengend Snippet: ( a ). Representative chronograms for DON-spiked wheat samples used to develop the ID-LFIA, retrieved in dissociation solution and analyzed by DART-QqQ-MS/MS, demonstrating the three distinct spiking levels in blue 0.5× TL, orange 1× TL, and green 1.5× TL. 13 C 15 DON is added as an internal standard before the MS/MS analysis. ( b ). Data analysis of 21 blank wheat samples, and spiked versions thereof in ID-LFIA/DART-QqQ-MS/MS, at three target level concentrations in blue 0.5× TL, orange 1× TL, and green 1.5× TL.

Article Snippet: For the screening assays, a smartphone-based LFIA kit, Rida Quick DON RQS Eco, including its dedicated DON extraction buffer, was obtained from r-Biopharm (Darmstadt, Germany).

Techniques: Tandem Mass Spectroscopy

Journal: Sensors (Basel, Switzerland)

doi: 10.3390/s21051861

Figure Lengend Snippet: ( a ). Chronogram of AcDON-spiked wheat sample used to develop the ID-LFIA and retrieved in dissociation solution analyzed by DART-QqQ-MS/MS, demonstrating the three distinct TL in blue 0.5× TL, orange 1× TL, and green 1.5× TL. 13 C 15 DON is added as an internal standard before the MS analysis. ( b ). Chronogram of the incurred corn starch sample used to develop the ID-LFIA and retrieved in dissociation solution analyzed in A . (+)DART-QqQ-MS/MS and B . (−)ESI-QqQ-MS/MS in flow injection analysis mode. 13 C 15 DON is added as an internal standard prior to MS analysis.

Article Snippet: For the screening assays, a smartphone-based LFIA kit, Rida Quick DON RQS Eco, including its dedicated DON extraction buffer, was obtained from r-Biopharm (Darmstadt, Germany).

Techniques: Tandem Mass Spectroscopy, Injection

Journal: Sensors (Basel, Switzerland)

doi: 10.3390/s21051861

Figure Lengend Snippet: Results from ID-LFIA/DART-QqQ-MS/MS analysis and the respective LFIA smartphone screening of AcDON-spiked wheat samples at three target levels and the analysis of an incurred corn starch sample.

Article Snippet: For the screening assays, a smartphone-based LFIA kit, Rida Quick DON RQS Eco, including its dedicated DON extraction buffer, was obtained from r-Biopharm (Darmstadt, Germany).

Techniques:

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dedicated don extraction buffer (R-Biopharm)

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Images

1) Product Images from "From Smartphone Lateral Flow Immunoassay Screening to Direct MS Analysis: Development and Validation of a Semi-Quantitative Direct Analysis in Real-Time Mass Spectrometric (DART-MS) Approach to the Analysis of Deoxynivalenol"

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